Correction to “Probing the Phosphopantetheine Arm Conformations of Acyl Carrier Proteins Using Vibrational Spectroscopy”
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چکیده
■ REFERENCES (1) Corrected ref 2: Nguyen, C.; Haushalter, R. W.; Lee, D. J.; Markwick, P. R. L.; Bruegger, J.; Caldara-Festin, G.; Finzel, K.; Jackson, D. R.; Ishikawa, F.; O’Dowd, B.; McCammon, J. A.; Opella, S. J.; Tsai, S.-C.; Burkart, M. D. Nature 2014, 505, 427. (2) Corrected ref 3: Masoudi, A.; Raetz, C. R. H.; Zhou, P.; Pemble, C. W., IV. Nature 2014, 505, 422. (3) Corrected ref 4: Dutta, S.; Whicher, J. R.; Hansen, D. A.; Hale, W. A.; Chemler, J. A.; Congdon, G. R.; Narayan, A. R. H.; Hak̊ansson, K.; Sherman, D. H.; Smith, J. L.; Skiniotis, G. Nature 2014, 510, 512. (4) Corrected ref 5: Whicher, J. R.; Dutta, S.; Hansen, D. A.; Hale, W. A.; Chemler, J. A.; Dosey, A. M.; Narayan, A. R. H.; Hak̊ansson, K.; Sherman, D. H.; Smith, J. L.; Skiniotis, G. Nature 2014, 510, 560.
منابع مشابه
Probing the Phosphopantetheine Arm Conformations of Acyl Carrier Proteins Using Vibrational Spectroscopy
Acyl carrier proteins (ACPs) are universal and highly conserved domains central to both fatty acid and polyketide biosynthesis. These proteins tether reactive acyl intermediates with a swinging 4'-phosphopantetheine (Ppant) arm and interact with a suite of catalytic partners during chain transport and elongation while stabilizing the growing chain throughout the biosynthetic pathway. The flexib...
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Acyl carrier proteins (ACPs) are central hubs in polyketide and fatty acid biosynthetic pathways, but the fast motions of the ACP's phosphopantetheine (Ppant) arm make its conformational dynamics difficult to capture using traditional spectroscopic approaches. Here we report that converting the terminal thiol of Escherichia coli ACP's Ppant arm into a thiocyanate activates this site to form a s...
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Acyl carrier proteins are critical components of fatty acid and polyketide biosynthesis. Their primary function is to shuttle intermediates between active sites via a covalently bound phosphopantetheine arm. Small molecules capable of acylating this prosthetic group will provide a simple and reversible means of introducing novel functionality onto carrier protein domains. A series of N-activate...
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The acyl carrier protein (ACP) requires posttranslational modification with a 4'-phosphopantetheine arm for activity, and this thiol-terminated modification carries cargo between enzymes in ACP-dependent metabolic pathways. We show that acyl-ACP synthetases (AasSs) from different organisms are able to load even, odd, and unnatural fatty acids onto E. coli ACP in vitro. Vibrio harveyi AasS not o...
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Protein dynamics plays an important role in protein function. Many functionally important motions occur on the microsecond and low millisecond time scale and can be characterized by nuclear magnetic resonance relaxation experiments. We describe the different states of a peptidyl carrier protein (PCP) that play a crucial role in its function as a peptide shuttle in the nonribosomal peptide synth...
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عنوان ژورنال:
دوره 136 شماره
صفحات -
تاریخ انتشار 2014